IRRI SERVICE LABORATORIES
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Macro and micronutrients by Inductively Coupled Plasma-Optical Emission Spectroscopy
Macro and micronutrients in rice grain is one of the important factors that affect quality. Different countries in the world face varying nutritional needs depending on their dietary preference, socioeconomic, and environmental condition. Breeding efforts are being made to address and improve rice nutritive value.
Inductively Coupled Plasma–Optical Emission Spectrometer (ICP-OES) is used to determine trace elements in the solution. All solid matrices need acid digestion prior to analysis. An internal standard could also be used and should be matrix-matched with the standards. If an internal standard is used, the instrument software should be programmed to correct intensity differences between samples and standards. The following analytes have been determined through this method: Al, Ca, Cu, Fe, Mg, Mn, Mo, P, K, Na, S, and Zn.
Note: To determine macro and micronutrients, the sample weight required is at least 4 g of milled rice or 3 g of rice flour placed in a paper bag. In order to keep the integrity of the customers' samples, senders are requested to place them in secure plastic boxes.
Boss CB, Fredeen KJ. 2005. Concepts, instrumentation, and techniques in Inductively Coupled Plasma Optical Emission Spectrometry. (3rd ed.)
Standard operating procedure analysis of major, minor, and trace elements in plant tissue samples with ICP-OES and ICP-MS. (2005). Soil and Plant Analysis Laboratory University of Wisconsin- Madison.
U.S. Environmental Protection Agency. Determination of trace elements in drinking water by axially viewed inductively coupled plasma-atomic emission spectrometry--method 200.5 Revision 4.8 October 2003 (EPA/600/R-06/115).
U.S. Environmental Protection Agency. SW-846 test methods for evaluating solid waste, physical/chemical methods. Chapter 1 Quality Control Revision 1 July 1992.
U.S. Environmental Protection Agency. SW-846 test methods for evaluating solid waste, physical/chemicals methods. Chapter 3 Inorganic Analytes Revision 4 Feb 2007.
U.S. Environmental Protection Agency. SW-846 test methods for evaluating solid waste, physical/chemicals methods. Inductively Coupled Plasma-Atomic Emission Spectrometry-- Method 6010C. Revision 3 February 2007.
WinLab32 instrument control software guide. 2006. Perkin Elmer.
Juliano, B. Rice Chemistry and Quality. Philippine Rice Research Institute, 2007.
Sreenivasulu, N. (2018). Rice Grain Quality: Methods and Protocols. New York, NY: Humana Press.