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Carbon-13 and Nitrogen-15 analysis by IRMS

Stable isotopes C-13 and N-15 in rice plant samples are analyzed using a flash elemental analyzer coupled to isotope ratio mass spectrometer (EA-IRMS). The technique involves the coupling of flash combustion with a gas chromatograph and isotope ratio mass spectrometer. The following analytes have been determined by this method: 15N atom % and 13C delta per mill.


Note: To determine 13C and 15N, the sample weight required is at least 3 g of plant sample.

References:
ISL standard operating procedure
ANCA-GSL user manual version 4.0 February 2001.
Estimation of measurement uncertainty in the determination of N-15 and C-13 in plant and soil samples. ISL-QMS. IRRI-IRS.